Journal: Photosynthesis Research

Article Title: State transitions in Physcomitrium patens studied with time-resolved fluorescence

doi: 10.1007/s11120-026-01206-4

Figure Lengend Snippet: A ) Decay associated spectra (DAS) of WT infiltrated with DCMU grown on minimal medium in 50 µmol m − 2 s − 1 or on minimal medium supplemented with 0.5% glucose in 17 µmol m − 2 s − 1 ( n = 3). B ) Area under the DAS in A) for every fluorescence lifetime ( n = 3), excitation wavelength 488 nm. The assignment of photosystems was done based on the fluorescence lifetimes. The stars (*) indicate statistical significance based on a one-way ANOVA followed by a Tukey post hoc test ( p < 0.05)

Article Snippet: The Chl fluorescence above 640 nm is spectrally separated with a grating of 150 g/mm (Shamrock, Andor).

Techniques: Fluorescence

Journal: Photosynthesis Research

Article Title: State transitions in Physcomitrium patens studied with time-resolved fluorescence

doi: 10.1007/s11120-026-01206-4

Figure Lengend Snippet: Decay associated spectra (DAS) of P. patens obtained with in vivo time-resolved fluorescence spectroscopy. A ) WT in dark-adapted state, excitation wavelength 488 nm ( n = 3), B ) WT in dark adapted state (same as A) and WT infiltrated with DCMU to close all PSII reaction centres, excitation wavelength 488 nm, C ), Isolated PSI-small rich fraction from a sucrose gradient ( n = 3), excitation wavelength 488 nm, D ), Normalized decay-associated spectra (DAS) of WT protonemata incubated with DCMU. In addition, the DAS of the isolated PSI complex from Fig. 1C is added. The shaded area shows the SE. To compare DAS from different conditions ( B ) the sum of the 3 DAS is normalized to the same area

Article Snippet: The Chl fluorescence above 640 nm is spectrally separated with a grating of 150 g/mm (Shamrock, Andor).

Techniques: In Vivo, Fluorescence, Spectroscopy, Isolation, Incubation

Journal: Photosynthesis Research

Article Title: State transitions in Physcomitrium patens studied with time-resolved fluorescence

doi: 10.1007/s11120-026-01206-4

Figure Lengend Snippet: A ) An example of a PAM state transition measurement in WT and stn7. The red bar indicates when the actinic light (3 µmol m -2 s -1 in this example) was on, and the dark-red bar indicates when the far-red light was on. The dots indicate the fluorescence intensity after a saturating pulse in state 1 (F m1 ) and state 2 (F m2 ). PAM measurements like in this example are used for calculating the parameters plotted in B ) and C ). B ) NPQ value of WT and stn7 measured at various actinic light intensities ( n = 8). NPQ values are calculated using the Stern-Volmer equation in the figure. C ) qT value of WT and stn7 measured at various actinic light intensities ( n = 8)

Article Snippet: The Chl fluorescence above 640 nm is spectrally separated with a grating of 150 g/mm (Shamrock, Andor).

Techniques: Fluorescence

Journal: Photosynthesis Research

Article Title: State transitions in Physcomitrium patens studied with time-resolved fluorescence

doi: 10.1007/s11120-026-01206-4

Figure Lengend Snippet: Schematic drawing of in vivo time-resolved fluorescence measurements to study state transitions. Protonemata (grown on minimal medium) are first measured in a dark-adapted state (state 1). Then an in vivo time-resolved fluorescence spectrum is measured after 15 min of acclimation to 3 µmol m -2 s -1 blue light (state 2) and again after 45 min of illumination with far-red light (state 1)

Article Snippet: The Chl fluorescence above 640 nm is spectrally separated with a grating of 150 g/mm (Shamrock, Andor).

Techniques: In Vivo, Fluorescence

Journal: Photosynthesis Research

Article Title: State transitions in Physcomitrium patens studied with time-resolved fluorescence

doi: 10.1007/s11120-026-01206-4

Figure Lengend Snippet: A ) Decay associated spectra (DAS) of WT obtained with in vivo time-resolved fluorescence spectroscopy after acclimation to 3 µmol m -2 s -1 blue or 3 µmol m -2 s -1 far-red light ( n = 4). B ) Decay associated spectra (DAS) of stn7 obtained with in vivo time-resolved fluorescence spectroscopy after acclimation to 3 µmol m -2 s -1 blue or 3 µmol m -2 s -1 far-red light ( n = 4), excitation wavelength 488 nm. The shaded area shows the SE. C ) Total PSI contribution of WT and stn7 after dark adaptation (DA) or adaptation to blue (BL) or far-red (FR) light. The sum under the 3 DASs is normalized to 1. The total PSI contribution is the sum of the area under the spectrum of the 35–40 ps component and the 170–190 ps component. The letters indicate statistical significance based on a one-way ANOVA followed by a Tukey post hoc test D ) PSI-large contribution of WT and stn7 after dark adaptation (DA) or adaptation to blue (BL) or far-red (FR) light. The PSI-large contribution is the area under the spectrum of the 170–190 ps component. The letters indicate statistical significance based on a one-way ANOVA followed by a Tukey post hoc test ( p < 0.05)

Article Snippet: The Chl fluorescence above 640 nm is spectrally separated with a grating of 150 g/mm (Shamrock, Andor).

Techniques: In Vivo, Fluorescence, Spectroscopy